| | Category | ME | L18 | Natural Cure for Breast Cancer |
| | Abstract | Breast cancer is the second leading cause of cancer deaths in women |
| | today and is the most common cancer among women. Epigallocatechin |
| | gallate in green tea has antioxidant properties. The aim of my project was |
| | to find out whether the naturally available EGCG (green tea extract) could |
| | kill breast cancer cells. I compared it to the known treatment, a |
| | chemotherapy agent, Faslodex(ICI 182780) anti-estrogen receptor blocking |
| | agent. |
| | |
| | I treated breast cancer cells with various amounts of EGCG (10µmol, |
| | 50µmol, 100µmol, 200µmol) and Faslodex (1nmol, 10nmol, 100nmol) using |
| | fetal bovine serum as a control medium. I incubated the cancer cells for 96 |
| | hours. I added Cell Counting Kit-8 (CCK-8) solution to determine the number |
| | of cells killed. The CCK-8 solution contains a WST-8 tetrazolium salt which |
| | is converted by dehydrogenase enzyme in living cells and forms |
| | formazan, a yellow colored dye. I used a spectrophotometer to detect the |
| | amount of light passing through the solutions at 450nm. The optical density |
| | obtained was varied by the amount of formazan produced. Optical density |
| | is directly proportional to the number of viable cells which was then |
| | calculated with a standard calibration curve. |
| | |
| | According to my research, 10µmol EGCG killed 15.28% cells, 50µmol EGCG |
| | killed 40.46% cells, 100µmol EGCG killed 45.78% cells, and 200µmol EGCG |
| | killed 79.33% cells. Further, 1nmol ICI killed 6.75% cells, 10nmol ICI killed |
| | 44.91% cells, and 100nmol ICI killed 58.57% cells. |
| | |
| | This concludes that green tea extract (EGCG) has a lethal effect on breast |
| | cancer cells compared to currently available Faslodex. |
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| | |
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| | |
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| | |
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| | )_in_green_tea.aspx>. |
| | |
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